Article Figures & Data
Figures
- Figure 1
Beta-diversity changes during 72 hours. A significant difference between the microbial community composition of critically injured patients from admission (0 hour), 24 or 72 hours after admission was observed when analyzing the weighted UniFrac matrices, as depicted in three-dimensional Principle Coordinates Analysis. This implies that there is both a phylogenetic and relative abundance component to the difference between the microbial communities.
- Figure 2
Microbial changes over time, depicted at order taxonomic level.
- Figure 3
Significantly enriched OTUs at 0 vs. 72 hours. Significantly enriched OTUs (p<0.05, q<0.05) were determined using negative binomial regression. When comparing the 0 and 72 hours samples there were 124 and 151 significantly enriched OTUs at each time point, respectively. OTU, operational taxonomic units
Tables
- Table 1
Patient demographics, injury characteristics, treatments and outcomes
Transfusion Antibiotics Age Sex Race ISS Mechanism 0 hour 24 hours* 72 hours‡ 0 hour 24 hours 72 hours TBI ICU Outcome 26 Female Asian 25 Blunt No Yes No No Yes No Yes 4 Alive 26 Male Asian 27 Blunt Yes Yes Yes No Yes Yes Yes 14 Alive 33 Male Latino 17 Blunt No No No No Yes Yes Yes 25 Alive 91 Male Asian 17 Blunt No No † No No † Yes 13 Alive 60 Male White 75 Blunt No No No No No No Yes 15 Dead 37 Male Latino 30 Blunt No No † No No † Yes 19 Alive 42 Male White 43 Blunt Yes Yes Yes No Yes Yes No 10 Alive 52 Male Latino 75 Blunt Yes Yes Yes No Yes Yes Yes 4 Dead 85 Male Asian 30 Blunt No Yes † No Yes † No 25 Alive 70 Male White 41 Blunt No Yes Yes No Yes Yes Yes 30 Alive 46 Male White 14 Penetrating No Yes Yes No Yes No No 8 Alive 20 Male Latino 21 Penetrating No Yes No No Yes Yes No 8 Alive *Product transfused in the 0 to 24-hour interval.
†No sample collected.
‡Product transfused in the 25 to 72-hour interval.
ICU, days in intensive care unit; ISS, Injury Severity Score; TBI, traumatic brain injury.
- Table 2
Measures of alpha-diversity in stool microbiome (p Values)
Evenness Richness Shannon
diversitySimpson diversity Inverse Simpson diversity Injured biome over time* 0.279 0.837 0.574 0.268 0.421 Uninjured vs injured biome† 0.148 0.390 0.167 0.261 0.136 *Stool microbiome of injured patients at 0 vs. 24 hours vs. 72 hours.
†Stool microbiome of uninjured vs. injured patients at 0 hour.
- Table 3
Measures of beta-diversity in stool microbiome (p Values)
Canberra Bray-Curtis Unweighted UniFrac Weighted UniFrac Injured biome over time* 0.627 0.179 0.681 0.023 Uninjured vs injured biome† 0.064 0.177 0.159 0.123 *Stool microbiome of injured patients at 0 vs. 24 hours vs. 72 hours.
†Stool microbiome of uninjured vs. injured patients at 0 hour.
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