Skip to main content

Main menu

  • Latest content
    • Latest content
  • Archive
  • About the journal
    • About the journal
    • Editorial board
    • Information for authors
    • FAQs
    • Thank you to our reviewers
    • The American Association for the Surgery of Trauma
  • Submit a paper
    • Online submission site
    • Information for authors
  • Email alerts
    • Email alerts
  • Help
    • Contact us
    • Feedback form
    • Reprints
    • Permissions
    • Advertising
  • BMJ Journals

User menu

  • Login

Search

  • Advanced search
  • BMJ Journals
  • Login
  • Facebook
  • Twitter
TSACO

Advanced Search

  • Latest content
    • Latest content
  • Archive
  • About the journal
    • About the journal
    • Editorial board
    • Information for authors
    • FAQs
    • Thank you to our reviewers
    • The American Association for the Surgery of Trauma
  • Submit a paper
    • Online submission site
    • Information for authors
  • Email alerts
    • Email alerts
  • Help
    • Contact us
    • Feedback form
    • Reprints
    • Permissions
    • Advertising
Open Access

Development of a large animal model of lethal polytrauma and intra-abdominal sepsis with bacteremia

Rachel L O'Connell, Glenn K Wakam, Ali Siddiqui, Aaron M Williams, Nathan Graham, Michael T Kemp, Kiril Chtraklin, Umar F Bhatti, Alizeh Shamshad, Yongqing Li, Hasan B Alam, Ben E Biesterveld
DOI: 10.1136/tsaco-2020-000636 Published 1 February 2021
Rachel L O'Connell
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Glenn K Wakam
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Glenn K Wakam
Ali Siddiqui
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aaron M Williams
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathan Graham
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael T Kemp
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiril Chtraklin
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Umar F Bhatti
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alizeh Shamshad
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yongqing Li
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hasan B Alam
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ben E Biesterveld
Surgery, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • eLetters
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Supplementary Materials
  • Additional Files
  • Figure 1
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1

    Rectus crush, abdominal injuries, femur fracture and traumatic brain injury. (A) Rectus abdominis separated from skin and subcutaneous tissue, exposing 5 cm of the muscle and crushed with a Kelly clamp to create a soft tissue injury. (B) Section of spleen 5 cm from its distal tip is transected sharply. Remaining spleen is oversewn to prevent excess blood loss. (C) Left median lobe of liver transected sharply 5 cm from its anterior tip. Cut edge of liver is allowed to hemorrhage freely for 30 seconds before being closed by oversewing the cut edge, cauterizing the surface, and packing with laparotomy pads. (D) 3 cm injury created in the sigmoid colon 10 cm proximal to the anterior peritoneal reflection, exposing fecal matter. The colotomy in left open. (E) Small cruciate incision is used to cut down onto the lateral femur. Femur fractured with .22 caliber bullet using a captive bolt gun, creating a long bone fracture. 4×4 gauze pads are placed into wound after fracture to prevent excess bleeding. (F) Close-up of the bolt gun used. (G) A 21 mm burr hole was made just anterior and to the right of the bregma to expose the dura for traumatic brain injury. A 5 mm burr hole was made anterior and left of the bregma for placement of an intracranial pressure monitor. (H) Controlled cortical impact device set up to deliver a 12 mm impact.

  • Figure 2
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2

    Experimental timeline. Invasive lines were placed (cannulation), followed by rectus muscle, bone, liver, spleen and colon injuries, then hemorrhage and traumatic brain injury (TBI). Animals were left in shock for 1–2 hours, depending on the model (survival animals had 2-hour shock, all other animals had 1-hour shock). Then treatment began with normal saline resuscitation given over 1 hour. Autologous packed red blood cells (pRBC) were given 2 hours after resuscitation. Concurrent with pRBC transfusion, the laparotomy was reopened; the colon injury was repaired and abdomen irrigated with normal saline; and antibiotics were administered. The animal was then monitored for an additional 4 hours at which point the experiment was ended for non-survival models.

  • Figure 3
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3

    Kaplan-Meier survival curves. X-axis shows hours since start of traumatic injuries.

  • Figure 4
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4

    Physiologic parameters. Heart rate (HR) in beats per minute (BPM), mean arterial pressure (MAP) in mm Hg, cardiac output (CO) in liters per minute (L/min) and central venous pressure (CVP) in mm Hg. X-axis shows hours since start of traumatic injuries.

Supplementary Materials

  • Figures
  • Additional Files
  • Supplementary data

    [tsaco-2020-000636supp001.pdf]

Additional Files

  • Figures
  • Supplementary Materials
  • Supplementary Data

    This web only file has been produced by the BMJ Publishing Group from an electronic file supplied by the author(s) and has not been edited for content.

    • Data supplement 1
  • Supplementary Data

    This web only file has been produced by the BMJ Publishing Group from an electronic file supplied by the author(s) and has not been edited for content.

    • Data supplement 1
PreviousNext
Back to top
Email

Thank you for your interest in spreading the word on TSACO.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Development of a large animal model of lethal polytrauma and intra-abdominal sepsis with bacteremia
(Your Name) has sent you a message from TSACO
(Your Name) thought you would like to see the TSACO web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Development of a large animal model of lethal polytrauma and intra-abdominal sepsis with bacteremia
Rachel L O'Connell, Glenn K Wakam, Ali Siddiqui, Aaron M Williams, Nathan Graham, Michael T Kemp, Kiril Chtraklin, Umar F Bhatti, Alizeh Shamshad, Yongqing Li, Hasan B Alam, Ben E Biesterveld
Trauma Surg Acute Care Open Jan 2021, 6 (1) e000636; DOI: 10.1136/tsaco-2020-000636

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Cite This
  • APA
  • Chicago
  • Endnote
  • MLA
Loading
Development of a large animal model of lethal polytrauma and intra-abdominal sepsis with bacteremia
Rachel L O'Connell, Glenn K Wakam, Ali Siddiqui, Aaron M Williams, Nathan Graham, Michael T Kemp, Kiril Chtraklin, Umar F Bhatti, Alizeh Shamshad, Yongqing Li, Hasan B Alam, Ben E Biesterveld
Trauma Surg Acute Care Open Jan 2021, 6 (1) e000636; DOI: 10.1136/tsaco-2020-000636
Download PDF

Share
Development of a large animal model of lethal polytrauma and intra-abdominal sepsis with bacteremia
Rachel L O'Connell, Glenn K Wakam, Ali Siddiqui, Aaron M Williams, Nathan Graham, Michael T Kemp, Kiril Chtraklin, Umar F Bhatti, Alizeh Shamshad, Yongqing Li, Hasan B Alam, Ben E Biesterveld
Trauma Surgery & Acute Care Open Jan 2021, 6 (1) e000636; DOI: 10.1136/tsaco-2020-000636
Reddit logo Twitter logo Facebook logo Mendeley logo
Respond to this article
  • Tweet Widget
  • Facebook Like
  • Google Plus One
  • Article
    • Abstract
    • Introduction
    • Methods
    • Results
    • Discussion
    • Data availability statement
    • Ethics statements
    • Footnotes
    • References
  • Figures & Data
  • eLetters
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Variability in firearm injury among major pediatric trauma centers across the USA
  • When public health crises collide: 5 years of pediatric firearm injury prevention opportunities
  • Effects of hemodilution on coagulation function during prolonged hypotensive resuscitation in a porcine model of severe hemorrhagic shock
Show more Original research

Similar Articles

 
 

CONTENT

  • Latest content
  • Archive
  • eLetters
  • Sign up for email alerts
  • RSS

JOURNAL

  • About the journal
  • Editorial board
  • Thank you to our reviewers
  • The American Association for the Surgery of Trauma

AUTHORS

  • Information for authors
  • Submit a paper
  • Track your article
  • Open Access at BMJ

HELP

  • Contact us
  • Reprints
  • Permissions
  • Advertising
  • Feedback form

©Copyright 2023 The American Association for the Surgery of Trauma