The biphasic opening of the blood–brain barrier in the cortex and hippocampus after traumatic brain injury in rats

doi:10.1016/S0304-3940(97)00239-5

Neuroscience Letters

Volume 226, Issue 1, 18 April 1997, Pages 33-36

https://doi.org/10.1016/S0304-3940(97)00239-5 Get rights and content

Abstract

This study examined the time course of the blood–brain barrier (BBB) opening and correlated this with brain edema formation after a lateral controlled cortical impact (CCI) brain injury in rats. Quantitative measurement of Evans blue (EB) extravasation using fluorescence was employed at 2, 4, 6 h and 1, 2, 3, 4 and 7 days after injury. Brain edema was measured by specific gravity of the tissue at corresponding time points. Two prominent EB extravasations were observed at 4–6 h and 3-day after injury in the injury-site cortex and the ipsilateral hippocampus. Brain edema became progressively more severe over time and peaked at 24 h after injury and began to decline after day 3. These results suggest that there is a biphasic opening of the BBB after CCI brain injury and the second opening of the BBB does not contribute to a further increase in edema formation.

Section snippets

Acknowledgements

This study was supported by funding from an NIH grant (PO1 NS 31220) and Department of Veterans Affairs. We carefully adhered to the animal welfare guidelines set forth in the `Guide for the Care and Use of Laboratory Animals', US Department of Health and Human Services, Pub. No. 85-23, 1985.

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The biphasic opening of the blood–brain barrier in the cortex and hippocampus after traumatic brain injury in rats

Abstract

Section snippets

Acknowledgements

Brain Res.

Neurosci. Lett.

Br. J. Anaesth.

Neurosci. Lett.

Blood–brain barrier breach following cortical contusion in the rat

J. Neurosurg.

Magnetic resonance imaging-monitored acute blood–brain barrier changes in experimental traumatic brain injury

J. Neurosurg.

Regional activity of ornithine decarboxylase and edema formation after traumatic brain injury

Neurosurgery

Protective effect of 5-lipoxygenase inhibitor AA 861 on cerebral edema after transient cerebral ischemia

J. Neurosurg.

Lateral cortical impact injury in rats: cerebrovascular effects of varying depth of cortical deformation and impact velocity

J. Neurotrauma

A controlled cortical impact model of traumatic brain injury in the rat

J. Neurosci. Methods

The role of oxygen radicals in traumatic injury: clinical implications

J. Emerg. Med.